Abstract
The extraction novocaine, lidocaine anestezina are studied from aqueous media by individual organic solvents (aliphatic alcohols, normal and isomeric structure of C 3 – C 9 alkylacetates C 2 – C 5 ) and their mixtures using of different salting-out agents (chlorides, sulfates, sodium and ammonium). The coefficients of the distribution and the degree of extraction of anesthetics are calculated. It is shown that with increasing number of carbon atoms in the molecules of alcohols and esters their extractant possibility is decreased and alcohols isomers are less effective than normal ones. For the most complete extraction of the anesthetics the mixtures of alcohols with isomeric structure and alkyl acetates are used. Two and ternary-component mobile phase at chromatography in thin layer are studied for separation of local anesthetics in the concentrates, the mobility coefficients of substances are calculated. Quantitative evaluation are carried out by application of specialized software. The separation coefficients of anesthetics are calculated. The technique of determination of analytes in aqueous media is developed. The results of the extraction- chromatographic determination of novocaine, lidocaine and anaesthesin in aqueous media are proved by method «introduced – found». The range of detectable concentrations is 0.1 – 1 mkg/sm 3 , detection limit is 0.02 mkg/sm 3 . The relative error of determination is less than 10 %. Keywords : local anesthetics, extraction, chromatography in a thin layer (Russian) DOI: http://dx.doi.org/10.15826/analitika.2013.17.4.013 Ia . I . Korenman , T . V . Chibisova , P . T . Sukhanov , M.V. Zybenko Federal State Budget Educational Institution of Higher Professional Education «Voronezh State University of Engineering Technologies» (FSBEI HPE «VSUET») , Voronezh, Russian Federation
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