Abstract
Unregulated use of antibiotics for the treatment of infectious diseases of bees can lead to their accumulation in beekeeping products, particularly in bee bread. The most effective methods for detecting residual amounts of antibiotics in bee bread are immuno-microchips technology, based on competitive chemiluminescent immunoassay and allowing semi-automated qualitative and quantitative analysis of a large number of samples. For determining the residual amounts of oxytetracycline in bee bread, a test system with scanning chemiluminometer of Randox evidence investigator was used. The sensitivity of the method to the antibiotic oxytetracycline was 5,0 μg/kg with a confidence of 95%; the whole analysis together with sample preparation takes 3...3,5 hours and does not require large expenditures of reagents. The method of immuno-microchips technology is highly sensitive and promising for study of bee products, particularly bee bread, for the presence of antibiotic residues.
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