Порівняння локального запалення при загостренні хозл та перитоніті з урахуванням цитокінового профілю

Abstract

Introduction. Pathological signs of chronic obstructive pulmonary disease (COPD) is the destruction of the parenchyma with emphysema and inflammation of the small airways. Peritonitis – one of the most frequent pathologies in surgery. It is associated with the development of inflammation on systemic and local levels. Determining the level of proinflammatory mediators in peritoneal fluid and serum and their ratio is important for the diagnosis and prognosis of disease and choice of effective treatments. Typically, the level of inflammatory cytokines in sputum and bronchoalveolar fluid of patients with COPD is increasing. On the contrary, the content of cytokine suppressive properties may decline. In literature there is no data on the comparative characteristics of proinflammatory cytokine suppressive and locally in exacerbations of COPD and development of serous-fibrinous peritonitis.The aim of the study – to compare local levels of proinflammatory cytokine suppressive and in patients with exacerbations of COPD and serous-fibrinous peritonitis.Methods of the research. The objects of the study were samples of sputum of COPD patients and control individuals and patients with peritoneal fluid serous-fibrinous peritonitis obtained during surgery. The content of cytokines in induced sputum and peritoneal fluid was determined by ELISA using reagents production "Vector-Best".Results and Discussion. It was found that the concentration of proinflammatory cytokines such as interlekiny-1β, - 6 (IL-1β, IL-6) and tumor necrosis factor-α (TNF-α), was significantly higher than in the control group, indicating the uniformity of local inflammation during peritonitis as well as in COPD. However, in patients with peritonitis content of IL-6 and TNF-α increased relative COPD patients, indicating a higher activity of local inflammation during peritonitis. Regarding suppressive cytokines, when peritonitis concentration of IL-10 was significantly higher, and transforming growth factor-β - lower than in patients with COPD. In serum and induced sputum of patients with COPD observed increase in IL-1ta TNF-α.Conclusions. Local inflammation in COPD exacerbation and serous-fibrinous peritonitis is characterized by the simultaneous increase in proinflammatory cytokines and suppressive. In patients with peritonitis compared to COPD exacerbation, observed a higher activity of local inflammation, manifested significant increase in the level of IL-6 and TNF-α. When analyzing the cytokine profile of the definition of proinflammatory mediators and suppressive installed uniformity of local inflammation in COPD exacerbation and development of serous-fibrinous peritonitis.