Abstract
The MTP4 (Metal Tolerance Protein 4) gene, which controls the resistance of sugar beet plants to heavy metals and is located on the 3rd chromosome has been studied. Micro clones grown in vitro with lethal Cd doses were used as explants. For PCR, specific primers MTP4A F/R and MTP4B F/R, covering the 2nd, 3rd and 4th exons and introns between them in the MTP4 gene, were used. Samples with resistance to heavy metals (in particular, Cd) contained some nucleotide changes in the 4th exon resulting in amino acid substitutions in the polypeptide chain. Based on the results of the bioinformatics analysis, it can be assumed with a high probability that the resistance of the studied breeding materials to heavy metals arose due to the presence of certain polymorphisms in the analyzed sugar beet gene. Geneious Prime software as a tool for the MTP4 gene sequence analysis will probably allow the in vitro selection of resistant forms for the subsequent use in the breeding scheme. Key words: sugar beet, МТР genes, genetic polymorphism, PCR, molecular genetic markers, breeding, DNA
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