Abstract
Aim: The aim of the study was to reveal the relationship between stress resistibility levels andsaliva microcrystallization and to investigate whether menstruation phase has infl uence on thesaliva microcrystalisation.Methods: The group of obseravtion included 26 healthly women aged 19-30. Facies of salivawere investigated by microscopic analysis of hydration of drops of mixed saliva (Havriluk, 2014).For estimation of stress resistibility levels questionnaire created by ISMA (International stressmanagement association, 2011) was used. To reveal the infl uence of menstrual phase on themicrocrystalisation saliva facies were collected from women every three days during the menstrualcycle.Results: It was discovered that the absence of microcrystalisation (4th type) was mostoften seen among people with low stress resistibility. What about relationship between salivamicrocrystalisation and menstrual cycle there was the trend of high incidence of 1st and 2ndtypes during the ovulation.Conclusions: Saliva investigation of microcrystallization types can be used as an additionalmethod of estimation of stress and as a method of the approximate detection of the ovulation, soit can be used for planning pregnancy. Method of evaluation of saliva microcrystallization types isa helpful tool for detection of characteristic of hormonal cycle and can be used for patients withsecondary amenorrhea, surgical menopause and prescription of hormonal replacement treatmentor specifi c treatment (e.g. chemotherapy).
Highlights
40 years ago among scientists awarded the Nobel Prize in Physiology or Medicine 1977 on «for their discoveries concerning the peptide hormone production of the brain» was Roger Guillmin
Aim: The aim of the study was to reveal the relationship between stress resistibility levels and saliva microcrystallization and to investigate whether menstruation phase has influence on the saliva microcrystalisation
For estimation of stress resistibility levels questionnaire created by ISMA (International stress management association, 2011) was used
Summary
40 years ago among scientists awarded the Nobel Prize in Physiology or Medicine 1977 on «for their discoveries concerning the peptide hormone production of the brain» was Roger Guillmin Selye’s PhD student in 1949-1953) who discovered of Corticotropin Releasing Factor, CRF, as key master hormone of stress reaction, and whole hypothalamic-pituitaryadrenal axis was identified. Based on this and plenty other additional observations, the basic and clinical research on stress is extensive and multidisciplinary in all areas of medicine, the tools of identification stress manifestation in humans is still limited [1, 2]. Determining personal stress level in real time could be of special interest in health monitoring, wellbeing and early prediction of stress-associated disorders. Recent data suggests that biofluids are very promised for health monitoring and creation non-invasive diagnostic tool for personal control and prevention decline of natural stress-resistibility. Recent data has shown that saliva secretome study is growing body of evidences for its using, as easy and early diagnostic tool [7, 8, 9, 10, 11]
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