Ответная реакция митохондрий тромбоцитов и лейкоцитов здоровых людей на воздействие микрочастиц атмосферного воздуха

Abstract

The influence of atmospheric microparticles on the state of human platelet and leukocyte mitochondria in healthy residents of the city of Vladivostok was studied. The state of mitochondria was assessed by changing the composition of membrane fatty acids and the inner mitochondrial membrane potential (∆Ψmito). The observation groups included 62 residents of Vladivostok and the Russian Islands at the age of 28 to 54 years. Vladivostok is taken as a territory with a high content of toxicants in the air of the micrometer range, island Russky - as the territory is most favorable for the environmental situation and the absence of air polluting microparticles. The fatty acid spectrum was studied by gas-liquid chromatography. The level of ∆Ψmito was studied ex tempore on flow cytofluorimeter. It was found that influence of atmospheric microparticles the accumulation of saturated fatty acids (12: 0, 14: 0, 16: 0, 18: 0) and n-6 polyunsaturated fatty acids (20: 3n-6, 20: 4n-6, 22: 4n-6), a decrease in n-3 polyunsaturated fatty acids and ∆Ψmito. The observed changes in the architecture of the inner mitochondrial membrane and its functional activity testify, on the one size, of the formation of compensatory reactions aimed at strengthening the lipid matrix of the membrane and reducing its permeability, on the other - on the development of mitochondrial dysfunction, the predisposition of cells to apoptotic changes. Identification of the spectrum of mitochondrial fatty acids membranes is an early indication of the disruption of cell functioning.