구강소독제의 적용 시간 및 기계적 세척 여부에 따른 타액 내 세균 수의 감소 효과

Abstract

Abstract Salivary Bacterial Counts on Application Time of Oral Antiseptic Agents and Mechanical Irrigation Hyoung-Sup Lim, Jae-Jin Kim 1 , Mija Kim 2 , Hak Kyun Kim 1 Department of Oral and Maxillofacial Surgery, School of Dentist ry, Chosun University, 1 Department of Oral and Maxillofacial Surgery, School of Medicine, Chungnam National University, Rese arch Institute for Medical Sciences, Chungnam National University, 2 Department of Nursing, Pai Chai UniversityPurpose: The purpose of this study is to establish the acceptable intraoral application time of antiseptic agents and evaluate the effect of mechanical irrigation. Methods: A total of 80 subjects were selected for this study. Saliva secreted at the resting state was taken. The subjects were divided into 8 experimental groups, and kept 10% povidone- iodine (PVI) or 0.2% chlorhexidine gluconate (CHX) for 20 or 40 seconds in their oral cavity with/without irrigation o f the oral cavity with sterilized normal saline, respectively. Then, the saliva was taken and diluted with phosphate buffered saline and then plated onto 5% sheep blood agar plates, which were incubated. Colony forming unit (CFU) was measured fo r the salivary bacterial counts.Results: After application of PVI and CHX, all the experimental groups showed statistically significant decrease in CFU (P＜0.01). Group 2 (PVI, 40 s) showed more significant reduction rate in C FU than group 4 (CHX, 40 s; P＜0.01). Group 6 (PVI, 40 s, irrigated) showed more significant reduction rate than gr oup 2 (PVI, 40 s; P＜0.01). Group 2 (PVI, 40 s) showed more significant reduction rate than group 1 (PVI, 20 s; P＜0.01). Conclusion: Application of PVI for 40 seconds and mechanical irrigation wi th sterilized normal saline showed the best result among the 8 groups in terms of the reduction rate of sal ivary bacterial counts.Key words:Povidone-iodine, Chlorhexidine, Irrigation, Bacteria