ИССЛЕДОВАНИЕ УСЛОВИЙ ФОРМИРОВАНИЯ РЕЗИСТЕНТНОСТИ У МИКРООРГАНИЗМОВ, ВСТРЕЧАЮЩИХСЯ В ГИНЕКОЛОГИЧЕСКОЙ ПРАКТИКЕ, К ЛЕКАРСТВЕННЫМ СРЕДСТВАМ СЕКНИДАЗОЛ И МЕТРОНИДАЗОЛ (IN VITRO)

Abstract

Bacterial vaginosis (BV) is a common disorder of the vaginal ecosystem in women of reproductive age and is associated with pelvic inflammatory disease and a range of pregnancy complications. In addition, BV is characterized by high rates of recurrence, and therefore, timely and accurate diagnosis and effective treatment methods are required.The purpose of the study: to study the antimicrobial action and the dynamics of the formation of resistance to metronidazole and secnidazole in microorganisms that cause BV. Materials and methods: the antimicrobial activity of the preparations was determined by the method of serial dilutions in broth. The dynamics of the formation of resistance to secnidazole and metronidazole was studied by passage cultures in a liquid nutrient medium with increasing concentrations of the antibiotic. Results: A study was made of the antibacterial activity of the drug secnidazole against the key pathogens of BV G. vaginalis and A. vaginae. It was shown that when compared with metronidazole traditionally used for the treatment of BV, secnidazole showed a higher level of activity against the studied bacterial species. An analysis of the dynamics of the formation of resistance to the drugs metronidazole and secnidazole in bacterial strains of the causative agent of BV showed that the most pronounced process of adaptation was observed in the A. vaginae strain to metronidazole. When developing resistance to secnidazole, the studied strains of G. vaginalis and A. vaginae did not acquire resistance to the test drug during long-term co-cultivation of bacteria in the presence of the drug. Conclusions: the results obtained indicate that secnidazole (Secnidox) has a low potential for the formation of resistance in G. vaginalis and A. vaginae with prolonged and repeated use of the drug. Secnidazole (Secnidox) may be highly effective in the treatment of BV caused by G. vaginalis and A. vaginae.