Особенности биологического действия низкоинтенсивного СВЧ-излучения на состояние противовирусной защиты клеток цельной крови при внебольничной пневмонии и у здоровых лиц

Abstract

The purpose of the study was to investigate the intracellular concentration factors of antiviral defense of cells whole blood of convalescents EAP and healthy individuals under the influence of low-intensity microwave irradiation of whole blood in vitro 1000 MHz. In accordance with the objectives of the study, in the lysates of mononuclear whole blood the authors determined by means of ELISAmethod the concentration of mitochondrial antiviral signaling protein MAVS, RIG-I-like receptor 3-type - helicase MDA-5, RIG-I-like receptor - helicase IFIH1, a transmembrane protein 173 (Tmeml73), interferon-regulated factors (IRF) 3, 7 and 8 subunits P50 and P65 nuclear transcription factor NF-kB, phosphorylated by serina in position 32 of the form of the inhibitor of nuclear transcription factor (ΙκΒ-α) and total concentrations. In addition, in the cell supernatant the authors assessed a spontaneous production by cells of the whole blood IFN-α, -|3.
 
 It was definite the ability of a single 20-minute microwave exposure to increase in the phase of convalescence EP intracellular level in mononuclear most important regulatory proteins - MDA5, MAVS, Tmeml73, IRF8. The irradiation stimulates the production of antiviral interferon beta. In the healthy individuals irradiation contributes largely to the increase in intracellular MAVS. A smaller effect is observed for MDA5, Tmeml73. This single microwave irradiation enhances production of both IFN-alpha and beta, encouraging a greater degree of the latest products. A feature of the biological effects of radiation is its immune-modulator effect on the intracellular content of studied mediators.